METHOD FOR THE RAPID DETERMINATION OF SUSCEPTIBILITY OR RESISTANCE OF BACTERIA TO ANTIBIOTICS

摘要

A method of rapidly evaluating the susceptibility of a strain of bacteria to a cell wall synthesis inhibiting antibiotic based on an assessment of cell enlargement in response to doses of the cell wall synthesis inhibiting antibiotic which are correlated to breakpoints of bacterial susceptibility.

主权项

1. A method of rapidly evaluating the susceptibility of an isolated strain of bacteria to a cell wall synthesis inhibiting antibiotic comprising:
a) establishing a bacteria culture from an isolated strain of bacteria; b) combining one or more doses of a cell wall synthesis inhibiting antibiotic to the bacteria culture, the concentration of each of the one or more doses being correlated to thresholds of antibiotic resistance for the isolated strain of bacteria to the cell wall synthesis inhibiting antibiotic; c) incubating the bacteria culture and the one or more antibiotic doses; d) assessing cell length or cell size of incubated bacteria for each of the one or more does of cell wall synthesis inhibiting antibiotic doses; and e) classifying the susceptibility of the isolated strain of bacteria to the cell wall synthesis inhibiting antibiotic based cell lengths or cells sizes of bacteria associated with each dose of the one or more doses of cell wall synthesis inhibiting antibiotic.