摘要 |
Disclosed is a method of preparing pure or substantially pure D-myo-inositol-3- phosphate from glucose-6-phosphate and/or fructose-6-phosphate. The method may also be applied to protected and/or derivative forms of glucose-6-phosphate and/or fructose-6-phosphate so as to form protected/derivative forms of D-myo-inositol-3- phosphate, for use in further chemical reactions. The enzyme D-myo-inositol-3- phosphate synthase (INO1) is contacted with the glucose-6-phosphate and/or fructose- 6-phosphate to generate labeled or unlabeled, protected or unprotected D-myo-inositol- 3-phosphate, which may be further reacted and/or purified. |