CRYSTAL STRUCTURE OF THE NanR AND ManNAc-6P COMPLEX, AND USES THEREOF

摘要

The present invention relates to a three-dimensional structure of a complex explored by crystallization of the complex of NanR which is a key pathogenic regulatory protein of Vibrio vulnificus and ManNA6P which is a NanR regulator. Further, the present invention relates to a modified NanR protein, a polynucleotide encoding the protein, a vector including the polynucleotide, and a transformant including the vector. Furthermore, the present invention relates to a method for screening a substance regulating interaction between NanR and the transcriptional control region of nan operon which is a gene cluster regulated by NanR, or a substance regulating interaction between NanR and ManNAc-6P, by designing three-dimensional structure of the complex, and to an antibacterial composition including the screened substance.

主权项

1. A method for crystallizing a complex of NanR protein and ManNAc-6P, comprising
(a) mixing NanR protein having an amino acid sequence of SEQ ID NO. 1 with N-acetylmannosamine 6-phosphate (ManNAc-6P); and (b) crystallizing the mixture of step (a).