发明名称 |
O-MANNOSYLTRANSFERASE DEFICIENT FILAMENTOUS FUNGAL CELLS AND METHODS OF USE THEREOF |
摘要 |
The present disclosure relates to compositions and methods useful for the production of heterologous proteins with reduced O-mannosylation in filamentous fungal cells, such as Trichoderma cells. More specifically, the invention provides a PMT-deficient filamentous fungal cell comprising a) at least a first mutation that reduces an endogenous protease activity compared to a parental filamentous fungal cell which does not have said first mutation, and, b) at least a second mutation in a PMT gene that reduces endogenous O- mannosyltransferase activity compared to a parental filamentous fungal cell which does not have said second mutation, wherein said filamentous fungal cell is selected from the group consisting of Trichoderma, Neurospora, Myceliophthora or Chrysosporium cell. |
申请公布号 |
CA2916594(A1) |
申请公布日期 |
2015.01.08 |
申请号 |
CA20142916594 |
申请日期 |
2014.07.03 |
申请人 |
NOVARTIS AG;GLYKOS FINLAND OY |
发明人 |
NATUNEN, JARI;HILTUNEN, JUKKA;HUUSKONEN, ANNE;SALOHEIMO, MARKKU;OSTERMEIER, CHRISTIAN;SOMMER, BENJAMIN PATRICK;WAHL, RAMON |
分类号 |
C07K14/37;C07K16/00 |
主分类号 |
C07K14/37 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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