Methods for Sequencing Nucleic Acid Molecules

摘要

Methods and compositions for determining the nucleic acid sequence of polynucleotides that are at least 1500 nucleotides in length are provided.

主权项

1. A method for making oligonucleotide fragments comprising
(a) cleaving a polynucleotide into a plurality of oligonucleotide sequences; (b) immobilizing only one end of each of the plurality of oligonucleotide sequences to a given attachment point on a first end of a substrate; (c) arranging the plurality of oligonucleotide sequences substantially linearly on the substrate; (d) attaching a label to a non-immobilized end of each of the plurality of oligonucleotide sequences; and (e) cleaving the plurality of oligonucleotide sequences at a cleavage line substantially perpendicular to said plurality of oligonucleotide sequences to generate a plurality of labelled oligonucleotide fragments.