| 发明名称 |
FLUORESCENCE OBSERVATION METHOD AND FLUORESCENCE OBSERVATION APPARATUS |
| 摘要 |
A fluorescence observation method of the present invention for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules includes: subjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules; and simultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light. |
| 申请公布号 |
US2015008340(A1) |
申请公布日期 |
2015.01.08 |
| 申请号 |
US201414495241 |
申请日期 |
2014.09.24 |
| 申请人 |
OLYMPUS CORPORATION |
发明人 |
FUJITA Katsumasa;NAGAI Takeharu;SAITO Kenta;YAMANAKA Masahito;TAKIMOTO Shinichi |
| 分类号 |
G01N21/64 |
主分类号 |
G01N21/64 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A fluorescence detection method for detecting plural types of fluorescence emitted from two or more kinds of fluorescent molecules, comprising
subjecting each of the two or more kinds of fluorescent molecules to multi-photon excitation by exciting light having an exciting wavelength equal to or shorter than 700 nm in a visible region, to generate fluorescence upon making use of an absorption wavelength band in a deep ultraviolet region of each of the two or more kinds of fluorescent molecules, and simultaneously detecting plural types of fluorescence generated on a shorter-wavelength side or on both of the shorter-wavelength side and a longer-wavelength side of the exciting wavelength of the exciting light. |
| 地址 |
Tokyo JP |
