发明名称 |
ON-COLUMN ENZYMATIC CLEAVAGE |
摘要 |
Herein is reported a method for obtaining a polypeptide by an immobilized metal ion affinity chromatography from a pro-polypeptide that comprise at its N- or C-terminus an metal ion affinity chromatography tag and a protease cleavage site comprising the step of recovering the polypeptide from the immobilized metal ion affinity chromatography column by incubating the bound pro-polypeptide with a protease, whereby the immobilized metal ion affinity chromatography material has been washed at least once with an urea solution. |
申请公布号 |
EP2820031(A1) |
申请公布日期 |
2015.01.07 |
申请号 |
EP20130706269 |
申请日期 |
2013.02.27 |
申请人 |
F.HOFFMANN-LA ROCHE AG |
发明人 |
FALKENSTEIN, ROBERTO;GROSSMANN, ADELBERT;HESSE, FRIEDERIKE |
分类号 |
C07K1/22;C07K14/47;C07K14/775;C07K17/06 |
主分类号 |
C07K1/22 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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