Assay device with optical filters and method of assaying

摘要

A device for testing an analyte comprises a pathway allowing passage of analyte from an application zone to a waste zone. The device includes label material that emits or modifies light and which binds to the analyte. Between the application and waste zones there is a capture zone having capture material for binding any analyte traversing the pathway to the pathway. A first optical filter on one surface of the device allows transmission of light emitted or modified by the label and blocks light of at least one other wavelength range. This enables the device to be illuminated from one surface and light emitted or modified by the label to be detected from the opposite surface. The device may include a second filter allowing shorter wavelength light to reach the label. The device may be viewed using an illuminating reader or held up to a light source for viewing.