| 发明名称 |
Populations of pancreatic progenitor cells and methods of isolating and using same |
| 摘要 |
A method of generating pancreatic progenitor cells is disclosed. The method comprises:
(a) differentiating stem cells under conditions such that at least a portion of the cells express glucose transporter 2 (GLUT2) so as to generate GLUT2-expressing cells; and(b) enriching for the GLUT2-expressing cells so as to generate a population of GLUT2 enriched cells, wherein at least 80% of the population of GLUT2 enriched cells express GLUT2, thereby generating pancreatic progenitor cells.;Isolated populations of cells generated according to the method, pharmaceutical compositions comprising same and uses thereof are also disclosed. |
| 申请公布号 |
US8927274(B2) |
申请公布日期 |
2015.01.06 |
| 申请号 |
US201113640532 |
申请日期 |
2011.04.12 |
| 申请人 |
Technion Research & Development Foundation Limited |
发明人 |
Itskovitz-Eldor Joseph;Fishman Bettina;Segev Hanna |
| 分类号 |
A61K35/39;A61K35/12;C12N5/071;A61P3/10;A61K38/28;A61K38/00 |
主分类号 |
A61K35/39 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method of generating GLUT2-expressing cells the method comprising:
(a) differentiating human stem cells under conditions such that at least a portion of said cells express glucose transporter 2 (GLUT2) on a membrane of said cells so as to generate GLUT2-expressing cells; and (b) isolating from said cells resultant of step (a) GLUT2-expressing cells using an anti GLUT2 antibody which binds to said GLUT2 expressed on said membrane so as to generate a population of GLUT2 enriched cells, wherein at least 80% of said population of GLUT2 enriched cells express GLUT2, thereby generating GLUT2-expressing cells. |
| 地址 |
Haifa IL |
