Compositions and methods for the detection of antibodies to native human leukocyte antigen

摘要

Provided herein are compositions comprising native and denatured human leukocyte antigens (HLA) and methods of making said compositions. Also provided herein are methods and kits for the detection of antibodies to native HLAs.

主权项

1. A composition comprising a solid substrate having linked thereto at least 90% purified native human leukocyte antigens and at most 10% purified denatured human leukocyte antigens produced by contacting the purified human leukocyte antigens with any one of trypsin-like clan protease, trypsin, chymotrypsin or elastase.