| 摘要 |
A method includes determining, with a computing device, one or more of a hue value, a saturation value, and an intensity value of a pixel in a region of interest of an image of a blood vessel sample. A physical response of the blood vessel sample to a vaso-active agent is determined based at least in part on one or more of the hue value, the saturation value, and the intensity value. |
| 主权项 |
1. A method comprising:
capturing a first image of a blood vessel sample at a time prior to application of a vaso-active agent to the blood vessel sample, wherein a timestamp is associated with the image; determining, by a computing device, a first hue value, a first saturation value, and a first intensity value for each of a first plurality of pixels in a region of interest of the first image of the blood vessel sample; capturing a plurality of subsequent images of the blood vessel sample during and after application of the vaso-active agent to the blood vessel sample, wherein a timestamp is associated with each subsequent image; determining, by the computing device, for each subsequent image, a subsequent hue value, a subsequent saturation value, and a subsequent intensity value for each of a plurality of pixels in the region of interest of the subsequent image of the blood vessel sample; determining, by the computing device, a first number of pixels that correspond to a lumen of the blood vessel sample based at least in part on the first hue value, the first saturation value, and the first intensity value of each of the first plurality of pixels in the region of interest of the first image; determining, by the computing device, a first area of the lumen of the blood vessel sample based on the first number of pixels and a known area of a single pixel; determining, by the computing device, for each of the plurality of subsequent images, a subsequent number of pixels that correspond to the lumen of the blood vessel sample based at least in part on the subsequent hue value, the subsequent saturation value, and the subsequent intensity value of each of the subsequent plurality of pixels in the region of interest of the subsequent image; determining, by the computing device, for each of the plurality of subsequent images, a subsequent area of the lumen of the blood vessel sample based on the subsequent number of pixels and the known area of the single pixel; determining, by the computing device, a physical response of the blood vessel sample to a vaso-active agent based at least in part on a comparison of the determined areas of the lumen of the blood vessel sample, wherein the physical response comprises a change in area of the lumen of the blood vessel sample; and plotting, by the computing device, the change in area of the lumen as a function of time. |
