Ovulation predictor test

摘要

The present invention is related to a diagnostic test kit for detecting luteinizing hormone (LH) in a biological sample at a concentration relative to a threshold concentration of LH. The device can include a release medium formed of a first material and including a labeled conjugate with a detectable label and a first binding member reactive with a first epitope of LH and a capture medium formed of a second, different material, in fluid communication with the release medium. The capture medium includes a result site having immobilized thereon a capture component capable of directly or indirectly binding LH that is bound to the labeled conjugate. The device is calibrated such that color development at the result site occurs only when the LH concentration of the liquid sample is greater than the threshold concentration.

主权项

1. A device for detecting the presence of an analyte in a biological sample comprising:
a. a release medium formed of a first material, wherein said release medium is configured to receive the biological sample; b. a capture medium located downstream from said release medium and in fluid communication with said release medium; c. a result site located on said capture medium; d. a scavenger binding member located on one of said release medium and said capture medium upstream from and separate from said result site, said scavenger binding member configured to directly or indirectly bind with a predetermined threshold concentration of the analyte; e. a labeled binding member reactive with a first epitope of the analyte to be detected in the biological sample, said labeled binding member being releasably disposed on said release medium; f. a second binding member capable of directly or indirectly binding to said analyte, said second binding member being disposed on one of said release medium and said capture medium; and g. a capture binding member is immobilized on said capture medium at said result site and is capable of directly or indirectly binding to one of said analyte and said second binding member, wherein detection of the analyte at said result site occurs only when the analyte concentration in the biological sample is greater than said predetermined threshold concentration.