| 摘要 |
<p>The present invention relates to genes and enzymes derived from Lactobacillus johnsonii PF01, and a method for extracting the enzyme for reducing hypercholesterolemia. More specifically, the present invention investigates characteristics of recombinant bshA, bshB, and bshC by amplifying the bshA, bshB, and bshC genes which code bile salt hydrolase derived from the Lactobacillus johnsonii PF01, cloning and overexpressing the same in Escherichia coli. The bile salt hydrolase bsh coded by the bshA, bshB, and bshC genes derived from the Lactobacillus johnsonii PF01 decomposes taurine binding and glycine binding of the bile salt, thereby confirming the fact that single strain of the Lactobacillus johnsonii PF01 has two kinds of bile salt hydrolases. The bile salt hydrolase bsh hereby: more efficiently lowers serum cholesterol levels in hypercholesterolemia patients using the efficacies of increasing intestinal viability and lowering cholesterol levels better than conventional bsh at the same time when adding the Lactobacillus johnsonii PF01 into foods and taking the same; and is useful for preventing people with normal cholesterol levels from having hypercholesterolemia.</p> |
