Fermentation medias and processes thereof

摘要

The present invention demonstrates the utility of carbonic acid amides such as urea or its derivatives, carbamates, carbodiimides & thiocarbamides as nitrogenous supplements in fermentation media for production of recombinant proteins to achieve enhanced bioconversion rates and peptides like insulin and insulin analogues, exendin and enzymes such as lipase using methanol inducible fungal expression systems such as Pichia.

主权项

1. A fermentation medium for production of a recombinant protein or a derivative or analog thereof by improving phosphate uptake through fermentation using a methanol inducible fungal species, said medium characterized in that a residual concentration of urea is maintained in a range of about 0.3M to about 1M, a methanol feeding rate ranges from about 6 g/L/h to about 20 g/L/h, basal salts per liter comprising:
Phosphoric acid (85%): from about 2.67 ml to about 133.5 mL, Calcium sulfate: from about 0.093 g to about 4.65 g, Potassium sulfate: from about 1.82 g to about 91 g, Magnesium sulfate-7H2O: from about 1.49 g to about 74.5 g, Potassium hydroxide: from about 0.413 g to about 20.65 g, Glycerol: from about 4 g to about 200 g;and
trace elements per liter comprising: Cupric sulfate.5H2O: from about 0.6 g to about 30 g, Sodium iodide: from about 0.008 g to about 0.4 g, Manganese sulfate-H2O: from about 0.3 g to about 15 g, Sodium molybdate-2H2O: from about 0.02 g to about 1 g, Boric Acid: from about 0.002 g to about 0.1 g, Cobalt chloride: from about 0.05 g to about 2.5 g, Zinc chloride: from about 2 g to about 100 g, Ferrous sulfate-7 H2O: from about 6.5 g to about 325 g, Biotin: from about 0.02 g to about 1 g, and Sulfuric Acid: from about 0.5 ml to about 25 ml.