Extended soluble PH20 polypeptides and uses thereof

摘要

Soluble PH20 polypeptides are provided, including extended soluble PH20 polypeptides, and uses thereof. Also provided are other C-terminally truncated PH20 polypeptides and partially deglycosylated PH20 polypeptides and uses thereof.

主权项

1. An extended soluble PH20 hyaluronidase (esPH20), wherein:
the esPH20 polypeptide exhibits hyaluronidase activity at neutral pH; the esPH20 polypeptide is secreted when expressed in a mammalian cell; the esPH20 polypeptide is soluble; and the esPH20 polypeptide is selected from among: a polypeptide that consists of the sequence of amino acids set forth as amino acid residues 36-495, 36-496, 36-497, 36-498, 36-499 or 36-500 of SEQ ID NO: 107; or a polypeptide that contains amino acid substitutions in the sequence of amino acids set forth as amino acids 36-495, 36-496, 36-497, 36-498, 36-499 or 36-500 of SEQ ID NO: 107, whereby the amino acid-substituted polypeptide consists of a sequence of amino acids that has at least 95% sequence identity with the corresponding sequence of amino acids set forth as amino acids 36-495, 36-496, 36-497, 36-498, 36-499 or 36-500 of SEQ ID NO: 107.