Organophosphorus hydrolase

摘要

The present disclosure provides methods, devices, systems and compositions for detecting and/or modifying chemical agents. In some embodiments, a biosensor may be configured to detect a chemical agent, modify that agent to a form with reduced toxicity, and/or detect the modified form of the chemical agent. The present disclosure also relates, in some embodiments, to variant organophosphorus hydrolase having one or more desirable amino acid substitutions.

主权项

1. An isolated organophosphorus hydrolase comprising a full-length amino acid sequence or a truncated amino acid sequence, truncated at an amino terminus of the sequence, containing the active site of Flavobacterium spp. Organophosphate hydrolase and the following set of substitutions as compared to wild type Flavobacterium spp. Organophosphate hydrolase: A80V, I106V, F132D, K185R, D208G, H257W, I274N, S308L, R319S.