Recombinant polypeptides of the members of the TNF ligand family and use thereof

摘要

The invention relates to polypeptides comprising, as constituent A, at least three monomers of a member of the TNF ligand family and, as constituent B, at least two peptide linkers that link the monomers of the member of the TNF ligand family to one another. The invention also relates to the use of these polypeptides for treating diseases and for producing a medicament or a vaccine. The invention also relates to methods for producing and isolating these polypeptides, to nucleic acids that code for these polypeptides, to vectors containing these nucleic acids, to host cells transfected with these vectors, and to pharmaceutical compositions containing these inventive objects. Finally, the invention relates to methods for the extracorporeal manipulation, depletion and/or removal of components contained in body fluids, e.g. by means of apheresis.

主权项

1. A method for extracorporeal depletion or removal of tumor necrosis factor receptor (TNFR) from blood or blood fractions comprising the following steps:
a) Optional separation of the blood into one or more blood fractions with solid or liquid components; b) Binding of the blood or the blood fractions expressing a TNFR to a surface or particle coupled to a polypeptide wherein the polypeptide comprises at least three components A and at least two components B, wherein each component A comprises a tumor necrosis factor (TNF) monomer or a functional fragment or a functional variant thereof, and each component B is a peptide linker under conditions allowing binding of TNFR in the blood or the blood fractions to the surface or the particle; and c) Separating the bound TNFR from the blood or the blood fractions, thereby depleting or removing TNFR from the blood or the blood fractions.