Sense oligonucleotide capable of controlling the expression of iNOS and composition comprising the same

摘要

The invention relates to sense oligonucleotide having a sequence complementary to a single-stranded RNA (antisense transcript) having a sequence complementary to mRNA of iNOS gene in order to control expression of iNOS (inducible nitric oxide synthase). The sense oligonucleotide of the present invention can control expression of iNOS and is useful for biological defense and treatment and prevention of diseases related to excessive production of NO, such as cancerogenesis, inflammatory disease, endotoxin shock by bacterial infection and the like.

主权项

1. A sense oligonucleotide, wherein said sense oligonucleotide is a synthetic single-stranded DNA oligonucleotide which comprises a sequence complementary to human iNOS (inducible nitric oxide synthase) mRNA antisense transcript, wherein said sequence complementary to human iNOS mRNA antisense transcript is complementary to at least 16 contiguous nucleotides of SEQ ID NO: 11, wherein said sense oligonucleotide is stabilized against decomposition by a nucleolytic enzyme, and wherein the stabilization is performed by a modification selected from the group consisting of LNA, PNA, ENA, and morpholino.