发明名称 |
Methods and compositions for dual extraction of protein and nucleic acid |
摘要 |
The present invention provides a method of isolating nucleic acid and protein from the same biological sample, comprising, in the following order: a) disrupting the biological sample; b) contacting the disrupted biological sample of (a) with a protein lysis buffer that lacks any component that denatures or reduces protein to produce a first lysate; c) centrifuging the first lysate of (b) to produce a first supernatant containing protein and a pellet containing nucleic acid; d) removing the first supernatant of (c), thereby isolating protein from the biological sample; e) contacting the pellet of (d) with nucleic acid lysis buffer to produce a second lysate; f) centrifuging the second lysate of (e) to produce a second supernatant containing nucleic acid; and g) removing the second supernatant of (f), thereby isolating nucleic acid from the same biological sample. |
申请公布号 |
US8927214(B2) |
申请公布日期 |
2015.01.06 |
申请号 |
US201213450039 |
申请日期 |
2012.04.18 |
申请人 |
Syngenta Participations AG |
发明人 |
Yarnall Michele Susan;Wang Wenling;Moore Karen;Fielder Mary |
分类号 |
C12Q1/68;C12P19/34 |
主分类号 |
C12Q1/68 |
代理机构 |
Myers Bigel Sibley & Sajovec, PA |
代理人 |
Myers Bigel Sibley & Sajovec, PA |
主权项 |
1. A method of isolating nucleic acid and undenatured and unreduced protein from the same biological sample, comprising, in the following order:
a) disrupting the biological sample, which comprises protein that is not denatured and not reduced; b) contacting the disrupted biological sample of (a) with a protein lysis buffer that lacks any component that denatures or reduces protein to produce a first lysate; c) centrifuging the first lysate of (b) to produce a first supernatant containing undenatured and unreduced protein and a pellet containing nucleic acid; d) removing the first supernatant of (c), thereby isolating undenatured and unreduced protein from the biological sample; e) contacting the pellet of (d) with nucleic acid lysis buffer to produce a second lysate; f) centrifuging the second lysate of (e) to produce a second supernatant containing nucleic acid; and g) removing the second supernatant of (f), thereby isolating nucleic acid from the same biological sample. |
地址 |
Basel CH |