FRET-labeled compounds and uses therefor

摘要

FRET-labeled compounds are provided for use in analytical reactions. In certain embodiments, FRET-labeled nucleotide analogs are used in place of naturally occurring nucleoside triphosphates or other analogs in analytical reactions comprising nucleic acids, for example, template-directed nucleic acid synthesis, DNA sequencing, RNA sequencing, single-base identification, hybridization, binding assays, and other analytical reactions.

主权项

1. A compound comprising a label portion and a reactant portion in an analytical reaction,
a) wherein the label portion comprises a FRET label; b) wherein the compound is configured such that the FRET label has an emission spectrum comprising at least two peaks that distinctly identify the reactant portion in the analytical reaction, wherein the reactant portion comprises a substrate for an enzyme.