Enzymatic oil-degumming method

摘要

A process of enzymatic degumming edible oils, comprising treating edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxyl group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprise one or more of the following amino acid sequences: SEQ ID NO: 1, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15, SEQ ID NO: 16, SEQ ID NO: 17, SEQ ID NO: 18, SEQ ID NO: 36, SEQ ID NO: 38, SEQ ID NO: 40, SEQ ID NO: 41, SEQ ID NO: 45, SEQ ID NO: 47, SEQ ID NO: 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID NO: 16 is also taught.

主权项

1. A method comprising:
enzymatically degumming an edible oil comprising: providing one or more lipid acyltransferases to the edible oil; transferring an acyl group from a phospholipid to one or more acyl acceptors selected from sterol and stanol to form one or more esters; wherein the one or more lipid acyltransferases have at least 5% acyltransferase activity, wherein: an edible oil to which no enzyme has been added is a control; (ii) in an enzymatic reaction with an edible oil, lipid material from the reaction and control are analyzed; (iii) acyltransferase activity is calculated as a percentage of total enzymatic activity using the formula:
(A×100)/((A+Δ% fatty acid)/Mv fatty acid), wherein: Δ% fatty acid=% fatty acid(enzyme)−% fatty acid(control); Mv fatty acid=average molecular weight of the fatty acids; and A=Δ% sterol ester/Mv sterol ester, wherein: Δ% sterol ester=% sterol/stanol ester(enzyme)−% sterol/stanol ester(control); and Mv sterol ester=average molecular weight of the sterol/stanol esters.