DETERMINATION OF CELL CHIRALITY AND DIAGNOSIS OF DISEASE THEREFROM

摘要

Methods for determining cell chirality using micropatterned substrates are disclosed. Also provided are methods for diagnosing diseases such as genetic diseases or cancer by comparing the chirality of sample cells from a subject with normal cells, and determining a difference in chirality between the sample cells and normal cells.

主权项

1. A method for determining chirality of a cell comprising:
(a) culturing a cell on a micropatterned substrate; wherein,
the micropatterned substrate comprises an x-axis, a y-axis, a path, an inner boundary, and an outer boundary;the path of the substrate micropattern is at least partially or substantially parallel with the y-axis;a surface of the path of the substrate micropattern is suitable for culture of the cell;the inner boundary or the outer boundary is at least partially or substantially perpendicular to the x-axis; and (b) determining directionality of the cell proximal to the inner boundary or the outer boundary; wherein
directionality of the cell comprises a leftward or a rightward migration of the cell over time or directionality of the cell comprises a leftward or a rightward alignment of the cell;directionality of the cell is measured along the y-axis with respect to the x-axis as measured from
(i) the path to the inner boundary along the x-axis;(ii) the path to the outer boundary along the x-axis;(iii) a nucleus of the cell to a centrosome of the cell along the x-axis; or(iv) the nucleus of the cell to a Golgi apparatus of the cell along the x-axis.