Detecting Chemical And Biological Agents Using Textile-Based Sensors

摘要

Methods and systems for detecting chemical and biological agents using oligonucleotide aptamers. A sensor includes a detection aptamer that has a binding domain for the chemical or biological agent, and is bound to fibers of a textile such as a patch or article of clothing. The detection aptamer can be stabilized and enhanced through a stabilization agent such as trehalose or through binding to a nanoparticle which is then bound to the fiber. Binding of the chemical and biological agent of interest to the detection aptamer can be reported to the user or wearer of the textile in a variety of ways, including visually and electrically.

主权项

1. A textile-based sensor for detecting the presence of a biological or chemical target, the sensor comprising:
a plurality of textile fibers; and a plurality of aptamer molecules, each comprising a target binding domain, immobilized to the plurality of textile fibers; a stabilizing agent to stabilize the plurality of aptamer molecules, wherein the stabilizing agent is selected from the group consisting of trehalose, a natural polymer, a synthetic polymer, and combinations thereof; wherein, in the presence of the target, the target binds to the target binding domain of one or more of the plurality of aptamer molecules and a reporter signal is generated by the sensor.