发明名称 |
NOVEL GENE DERIVED FROM MUD FLAT METAGENOME AND NOVEL PROTEIN OBTAINED THEREFROM SHOWING COACTIVITY OF PHOSPHOLIPASE AND LIPASE |
摘要 |
The present invention relates to a novel gene derived from a tidal flat metagenome, and a novel protein obtained therefrom showing the coactivity of phospholipase and lipase. Specifically, the novel gene isolated from the metagenome library of tidal flat sedimentsand the protein having phospholipase and lipase activities encoded from the novel gene: are expressed in a water-soluble form to be mass-producible; enable ultra high-purity protein to be obtained through single step purification using an Ni-NTA column; show good activity in the pH range of 5 ˆ¼ 10; maintain good low temperature activity and stability up to a temperature of 3°C to 40°C; and have high resistance against various organic solvents. Therefore, the novel gene and the protein can be usefully used for various industrial fields such as the purification and conversion of oil and fat, bio-medicine, and fine chemistry. |
申请公布号 |
EP2784160(A4) |
申请公布日期 |
2014.12.31 |
申请号 |
EP20120850464 |
申请日期 |
2012.07.17 |
申请人 |
KOREA RESEARCH INSTITUTE OF BIOSCIENCE AND BIOTECHOLOGY |
发明人 |
YOON, JUNG-HOON;LEE, MI HWA;KANG, CHUL HYUNG;OH, KI HOON;OH, TAE KWANG |
分类号 |
C12N15/55;C07K14/195;C12N9/20;C12N15/63;C12P21/00 |
主分类号 |
C12N15/55 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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