Composition comprising an isolated polysaccharide comprising a beta 1,6-glucosamine polymer, the composition comprising the isolated polysaccharide conjugated to a carrier compound, a method of making the isolated polysaccharide, a pharmaceutical composition, use of the isolated polysaccharide in the preparation of a medicament, a method for generating antibodies and a method of identifying a monoclonal antibody

摘要

The invention relates to compositions of a deacetylated poly N-acetylated glucosamine (dPNAG) of Staphylococci. The dPNAG may be isolated from natural sources or synthesized de novo. The invention also relates to the use of dPNAG as a vaccine for inducing active immunity to infections caused by Staphylococcus aureus, S. epidermidis, other related coagulase-negative or coagulase-positive Staphylococci, and other organisms carrying the ica (intracellular adhesion) locus. The invention further provides methods of use for antibodies directed to dPNAG, particularly for inducing passive immunity to the same class of infections.