Method for acquiring genetically identical gamete from lethal fish haploid-derived germ cell via germ line chimera

摘要

Disclosed are a method for acquiring a germ line chimeric fish having fish haploid germ cells, a germ line chimeric fish having haploid germ cells obtained by the aforesaid method, and a genetically identical gamete, said gamete having been derived from a donor haploid germ cell, produced by a germ line chimeric fish obtained by the aforesaid method.

主权项

1. A method for acquiring a chimeric male fish having a haploid germ cell, said male fish being selected from the group consisting of loach, goldfish and zebrafish, comprising the steps of:
a) genetically inactivating a goldfish or zebrafish egg or a goldfish or zebrafish sperm, followed by insemination to provide a haploid donor fertilized egg; b) obtaining a fertilized egg of a host goldfish or zebrafish; and c) transplanting donor primordial germ cells obtained from a haploid donor embryo developed from the haploid donor fertilized egg into a host embryo derived from the host fertilized egg, thereby acquiring the chimeric male fish having the haploid germ cells.