Methods and devices to generate luminescence from integrated electrode chips by cathodic and bipolar pulses

摘要

The invention describes novel chemiluminescence electrode devices and their novel properties to enable achieving luminescence signal by electrical excitation by cathodic or bipolar pulses in aqueous electrolyte solutions. These devices form a significant improvement in construction of cheap and reliable means for especially diagnosis of health conditions in point-of-need purposes.

主权项

1. An electrochemiluminescent analytical device to determine an amount of an analyte, said device comprising electrode chip and a hot electrochemiluminescense instrument (HECL), wherein:
a working electrode serving as a cathode and a counter electrode serving as an anode are integrated on the chip, and the anode and cathode are connected to the HECL via pads providing an electronic contact; the anode and cathode are constructed on same substrate, said substrate providing a basis of an electrode chip and said substrate being made of an insulating material or partially of conducting and partially of insulating material; the anode and the cathode being made from aluminum or silicon, and the anode successively comprising an insulating film of a thickness of at least 100 nm and coated with an oxide layer of 0.5-50 nm and a carbon paste or silver ink layer, and the cathode being successively coated with an oxide layer 0.5-20 nm and an insulating organic film of 0.5 to 20 nm; wherein during or after applying cathodic or bipolar pulses to the device from the HECL instrument a luminescence signal can be read from the device during, said signal being proportional to the amount of the analyte.