发明名称 |
FLUOROIMMUNOASSAY METHOD |
摘要 |
An object is to provide an immunoassay method requiring neither a solid-phase immobilization step nor a washing step, enabling quick and simple quantitative measurement of a target substance in a liquid phase and capable of visualizing an antigen. Such an object is attained by measuring the concentration of a target antigen present in a test substance by sequentially performing a step (a) of bringing an antibody light-chain variable region polypeptide and an antibody heavy-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; or bringing an antibody heavy-chain variable region polypeptide and an antibody light-chain variable region polypeptide labeled with a fluorescent dye into contact with an antigen in a test substance in a liquid phase; a step (b) of measuring the fluorescence intensity of the fluorescent dye; and a step (c) of computationally obtaining the level of the antigen contained in the test substance with reference to a positive correlation between the concentration of the antigen in a liquid phase and the fluorescence intensity of the fluorescent dye. |
申请公布号 |
CA2780845(C) |
申请公布日期 |
2014.12.30 |
申请号 |
CA20102780845 |
申请日期 |
2010.11.19 |
申请人 |
PROTEINEXPRESS CO., LTD. |
发明人 |
UEDA, HIROSHI;ABE, RYOJI;IHARA, MASAKI;TAKAGI, HIROAKI |
分类号 |
G01N33/533;C07K16/18;G01N21/78;G01N33/531 |
主分类号 |
G01N33/533 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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