Method for generating microscopic patterns of protein and other macromolecules

摘要

Methods and apparatuses for generating microscopic patterns of macromolecules such as proteins on a solid surface are described. Pulsed laser light is used to alter surface portions of a solid surface substrate in a predetermined pattern, by removing macromolecules from surface portions of the substrate where the light is focused. The same wavelength light at lower intensity can be used to visualize the removal by its reflection from the specimen surface along the path to the detector. Select macromolecules introduced to the substrate selectively adhere to select surface portions, thereby depositing macromolecules in the predetermined pattern on the solid surface.

主权项

1. A method of creating a micropattern of macromolecules on a substantially planar substratum, comprising:
providing a substantially planar substratum comprising a coating of adhesive molecules; binding a plurality of macromolecules to the coating of adhesive molecules; and applying incident multiphoton pulsed infrared laser light to one or more selected surface portions of the substantially planar substratum at an intensity sufficient for rebindably unbinding macromolecules from the selected surface portions, thereby creating a micropattern of macromolecules.