Phospholink nucleotides for sequencing applications

摘要

The present invention provides labeled phospholink, nucleotides that can be used in place of naturally occurring nucleotide triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays, and others.

主权项

1. A composition, comprising a labeled phospholink nucleotide, wherein said labeled phospholink nucleotide comprises a structure that has formula:wherein
B is a nucleobase; S is a sugar moiety; L is a bead comprising a scattering moiety or a magnetic moiety; R1 is selected from oxygen and sulfur; R2 is a linker moiety; and n is an integer selected from 0 to 9, wherein said bead is encapsulated in an organic or inorganic material.