Method and culture device for detecting yeasts and molds

摘要

A thin film culture device for detecting yeast and mold microorganisms in a sample is provided. The culture device comprises a body comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents. The plurality of indicator agents comprises three indicator agents for detecting distinct glycosidase enzyme activities, an indicator agent for detecting an alkyl esterase enzyme activity, and an indicator agent for detecting a phosphatase enzyme activity, wherein each of the plurality of indicator agents comprises a detectable reporter group. A method of using the culture device is also provided.

主权项

1. A culture device, comprising:
a body member comprising a self-supporting substrate having a first major surface and a second major surface; a first adhesive composition disposed on a portion of the first major surface of the substrate; a substantially dry, cold-water-soluble first hydrogel-forming composition adhered to the first adhesive composition; and a plurality of indicator agents disposed in the first adhesive composition, the plurality of indicator agents comprising:
three indicator agents for detecting distinct glycosidase enzyme activities;
wherein the at least three indicator agents for detecting distinct glycosidase enzyme activities comprise 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside, 5-bromo-4-chloro-3-indolyl-alpha-D-glucopyranoside, and 5-bromo-4-chloro-3-indolyl-beta-D-glucopyranoside;an indicator agent for detecting an alkyl esterase enzyme activity;
wherein the indicator agent for detecting an alkyl esterase enzyme activity comprises 3-indolyl-acetate; andan indicator agent for detecting a phosphatase enzyme activity;
wherein the indicator agent for detecting a phosphatase enzyme activity comprises 5-bromo-4-chloro-3-indolyl-phosphate; wherein each of the plurality of indicator agents comprises a detectable reporter group.