Systems and methods for high speed array printing and hybridization

摘要

Novel and improved systems and methods for high speed arraying, hybridization, quantitative development and/or assaying are provided. Some embodiments provide a web based arraying format. Some other embodiments provide a sheet based arraying format. Some embodiments use a drop on drop assaying or hybridization mode. In some embodiments, a substantially inert substrate is utilized. In some other embodiments, an interactive substrate is utilized.

主权项

1. A high speed array manufacturing system, comprising:
a series of dispense heads with each comprising a plurality of dispense channels with each dispense channel comprising a dispense tip to form a set of dispense tips, the dispense tips of each dispense channel being arranged in a predetermined configuration and having a predetermined spacing therebetween; a motion controls means that is configured to provide substantially continuous relative motion between a web-based substrate structure, having a plurality of fiducials and a longitudinal axis, and the dispense tips at a predetermined high speed in a direction generally parallel to the longitudinal axis; each set of the dispense tips being arranged in a staggered arrangement such that the predetermined configurations of the dispense tips are angled, diagonal or non-linear relative to said relative motion; a controller configured to selectively trigger each of the dispense channels based on a timing sequence and positioning of each fiducial relative to each dispense tip to dispense reagent droplets onto the substrate structure; wherein when the system is in use said drops comprise a part of the system; wherein said web-based substrate structure comprises a part of the system; and wherein the controller is further configured to control the operation and movement between the dispense tips and the substrate structure based on at least one control signal to control dispensing from the dispense channels relative to the substrate fiducials to form at least one array of reagent dots or spots, in a predetermined pattern, with the reagent dots or spots arranged at a predetermined location on the substrate structure to form a plurality of independent arrays on the substrate structure.