Vegetable oils and uses therefor

摘要

The specification relates to plants and their seeds and oil obtained therefrom, and to methods of producing same comprising oil having modified fatty acid compositions, such that 28% to 80% of the total fatty acid content in the seedoil is palmitic acid, 0% to 16% is palmitoleic acid, 0% to 4% is C16:2 fatty acid, 3% to 33% is stearic acid, 1% to 40% is oleic acid, 4% to 50% is linoleic acid and 0% to 10% is linolenic acid. The specification describes nucleic acid molecules encoding RNA capable of conferring these properties, in particular, RNA that inhibits expression of an oil biosynthesis gene encoding KASII in seeds of a plant. Genetic constructs and cells comprising the nucleic acid molecules are also described and claimed.

主权项

1. A process for producing seed oil, comprising the steps of:
(i) obtaining cotton seed, wherein the cotton seed has a reduced level relative to wild-type seed of a protein having amino acids in a sequence that is at least 95% identical to either the amino acid sequence set forth as SEQ ID NO: 2 or SEQ ID NO: 4, wherein said reduction is achieved by directly targeting a gene encoding said protein or a RNA product of said gene, and wherein the cotton seed is capable of producing a male-fertile cotton plant, wherein in the oil of the seed 28% to about 80% by weight of the total fatty acid content is C16 fatty acid, about 3% to about 33% is stearic acid, about 1% to about 40% is oleic acid, about 4% to about 50% is linoleic acid, and 0% to about 10% is linolenic acid; (ii) extracting the oil from the seed; and (iii) recovering the seed oil.