Device and method for electroporation-based delivery of molecules into cells and dynamic monitoring of cell responses

摘要

The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.

主权项

1. A method of monitoring a cellular response in real time comprising:
a. introducing a cell or cell population to a substrate comprising impedance monitoring electrodes; b. transfecting the cell or cell population with a molecule using a transfection method selected from the group consisting of a viral transfection method, a chemical transfection method, and a thermal transfection method; wherein the molecule is capable of effecting at least one selected from the group consisting of a cellular function, cell morphology, a cellular receptor, and a signal transduction pathway activated by a receptor; further wherein the cellular function is selected from the group consisting of cell proliferation cell adhesion and cell spreading; and c. monitoring impedance of the cell or cell population when the cell or cell population is in contact with at least one of the impedance monitoring electrodes.