| 摘要 |
The present invention relates to a method for preparing a fibrinogenase complex using endo-β-1,4-glucanase E, xylanase B, and mini cellulose binding protein A derived from Clostridium sp. microorganism. More specifically, the present invention relates to a method for preparing (i) a chimeric endo--β-1,4-glucanase E-mini cellulose binding protein A (cCelE-mCbpA) complex, (ii) a xylanase B- mini cellulose binding protein A (XynB-mCbpA) complex, and (iii) a chimeric endo--β-1,4-glucanase E- xylanase B- mini cellulose binding protein A (cCelE-XynB-mCbpA) complex by culturing a recombinant yeast having an ability to produce chimeric Endo��beta;��,4��lucanase E (cCelE), a recombinant yeast having an ability to produce xylanase B (XynB), and a recombinant yeast having an ability to produce mini cellulose binding protein A (miniCbpA). Also, the present invention relates to a method for manufacturing bioethanol using the same. |
