发明名称 |
METHOD FOR ASSESSING LOCAL HUMORAL ANTICHOLERAIC IMMUNITY IN VITRO SIMULATED IN EXPERIMENTAL ANIMALS |
摘要 |
FIELD: medicine.SUBSTANCE: method involves immunising with 5×10microbial cells Vibrio cholerae 5879; 10 days later, a common pool of anticholeraic Ig is recovered from enteric washing liquid. That is combined with producing the primary enterocyte culture from intact mice. Thereafter, the doubly-diluted anticholeraic Ig are applied in an amount of 3 ml on a monolayer of erythrocytes formed in wells of culture panels. The latter are added with alive choleraic vibrions of the strain Vibrio cholerae 5879, 40 m.c. per 1 erythrocyte and incubated for 3 hours. Then, the panels are washed three times, fixed with ethanol for 20 minutes and stained by Romanowsky-Giemsa. Local humoral anticholeraic immunity is assessed under the microscope by the number of vibrions stained in dark blue and attached to one erythrocyte.EFFECT: method enables assessing specific local humoral immunity and intensifying the anti-adhesive activity of the common pool of anticholeraic immunoglobulin.4 cl, 2 ex, 2 tbl |
申请公布号 |
RU2536707(C1) |
申请公布日期 |
2014.12.27 |
申请号 |
RU20130138025 |
申请日期 |
2013.08.13 |
申请人 |
FEDERAL'NOE KAZENNOE UCHREZHDENIE ZDRAVOOKHRANENIJA "ROSTOVSKIJ-NA-DONU ORDENA TRUDOVOGO KRASNOGO ZNAMENI NAUCHNO-ISSLEDOVATEL'SKIJ PROTIVOCHUMNYJ INSTITUT" FEDERAL'NOJ SLUZHBY PO NADZORU V SFERE ZASHCHITY PRAV POTREBITELEJ I BLAGOPOLUCHIJA CHELOVEKA |
发明人 |
OMEL'CHENKO NATAL'JA DMITRIEVNA;IVANOVA INNA ALEKSANDROVNA;TELESMANICH NATAL'JA ROBERTOVNA;BESPALOVA IRINA ALEKSANDROVNA;KISELEVA ALLA KONSTANTINOVNA |
分类号 |
G09B23/28;A61K38/08;A61P31/04;G01N33/531 |
主分类号 |
G09B23/28 |
代理机构 |
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代理人 |
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地址 |
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