MODIFIED BACTERIAL PATHOGENS AND METHODS FOR EFFECTUATING RAPID RESPONSE TO CONTAMINATION BY KNOWN OR UNKNOWN BACTERIAL PATHOGENS

摘要

Stable, constitutively expressed, chromosomal fluorescent transcriptional fusions in bacterial pathogens and methods of using the same to screen candidate compounds for anti-bacterial efficacy.

主权项

1. A method of screening candidate compounds for possessing anti-bacterial efficacy suitable for rapid response to contamination by a bacterial pathogen, the method comprising:
providing a modified bacterial pathogen, wherein the modification comprises a vector plasmid insert, said vector plasmid insert engineered to constitutively express a chromosomal transcriptional fusion fluorescent protein that results in sustained fluorescence while the modified bacterial pathogen is alive; contacting a candidate compound with the modified bacterial pathogen; determining whether the modified bacterial pathogen exhibits fluorescence; and identifying a candidate compound as comprising anti-bacterial efficacy where the fluorescence is extinguished.