| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method for producing higher yields of the form which binds with high affinity, while minimizing the presence of the lower affinity form.SOLUTION: The present invention relates to methods for the expression of high yields of the form of protein-Ig fusions having high affinity binding to its ligand by culturing hosts transformed with DNA encoding the desired fusions in a culture system at low temperature, thereby minimizing the amount of misfolded or misbridged protein forms. Host cells may be a transformed mammalian cell, an insect cell, a yeast cell, or a bacterial cell. Protein-immunoglobulin fusions may comprise TNF family or TNF receptor family member, for example, a lymphotoxin-βor lymphotoxin-βreceptor. This invention relates to pharmaceutical compositions comprising the protein-immunoglobulin fusion.</p> |
