METHOD FOR CULTURING ISLET CELLS AND METHOD FOR PREPARING CARRIER FOR ISLET CELL TRANSPLANTATION USING ATELOCOLLAGEN, AND ARTIFICIAL PANCREAS PREPARED USING SAME

摘要

The present invention provides a method of culturing pancreatic islet cells using a cationized atelocollagen prepared by ionization of high purity atelocollagen, a method of preparing a carrier for pancreatic islet cell transplantation using a cationized atelocollagen, and an artificial pancreas prepared using the same. According to the present invention, it is possible to increase the viability and/or glucose-dependent insulin secretion of pancreatic islet cells during culture of the cells by the use of a cationized atelocollagen or crosslinked atelocollagen scaffold obtained by ionization of high purity atelocollagen. In addition, it is possible to increase the viability and glucose-dependent insulin secretion of cultured and transplanted pancreatic islet cells by the use of a highly stable carrier for pancreatic islet cell transplantation that comprises cationized atelocollagen and alginate.

主权项

1. A method for preparing a carrier for pancreatic islet cell transplantation, the method comprising:
(a) mixing a cationized atelocollagen solution with an alginate solution to prepare a mixed solution; (b) adding pancreatic islet cells to the mixed solution of step (a); (c) allowing the pancreatic islet cells to be mixed with and surrounded by the mixed solution of step (a) to form a pancreatic islet cell complex comprising the pancreatic islet cells surrounded by the mixed solution of step (a); and (d) immersing the pancreatic islet cell complex obtained by step (c) comprising the pancreatic islet cells surrounded by the mixed solution of step (a), in a chelating agent solution to chelate the cationized atelocollagen and the alginate in the mixed solution and to produce a cationized atelocollagen/alginate bead containing the pancreatic islet cells therein.