SOYBEAN RESISTANT TO CYST NEMATODES

摘要

A transgenic soybean plant or parts thereof, resistant to soybean cyst nematodes, transformed to express Glyma18g02570, Glyma18g02580, or Glyma18g02590, or a variant thereof. Also provided is a method of making such a plant. Also provided is an artificial DNA construct encoding Glyma18g02570, Glyma18g02580, or Glyma18g02590, or a variant thereof.

主权项

1. A transgenic soybean resistant to soybean cyst nematode (SCN), or a seed, plant part, or progeny thereof, the soybean plant transformed with an artificial DNA construct comprising, as operably associated components in the 5′ to 3′ direction of transcription:
(a) a promoter that functions in a soybean; (b) a transcribable nucleic acid molecule comprising
(i) a nucleotide sequence selected from the group consisting of SEQ ID NO: 1 (Glyma18g02570), SEQ ID NO: 2 (Glyma18g02580), and SEQ ID NO: 3 (Glyma18g02590), or a nucleotide sequence at least 95% identical thereto encoding a polypeptide having Glyma18g02570, Glyma18g02580, or Glyma18g02590 activity, respectively;(ii) a nucleotide sequence encoding a polypeptide comprising SEQ ID NO: 4 (Glyma18g02570), SEQ ID NO: 5 (Glyma18g02580), SEQ ID NO: 6 (Glyma18g02590), or SEQ ID NO: 7 (Forrest SNAP A111D mutant), or an amino acid sequence at least 95% identical thereto having Glyma18g02570, Glyma18g02580, Glyma18g02590, or SNAP activity, respectively;(iii) a nucleotide sequence that hybridizes under stringent conditions to a nucleic acid sequence of SEQ ID NO: 1, SEQ ID NO: 2, or SEQ ID NO: 3, wherein the polynucleotide encodes a polypeptide having Glyma18g02570, Glyma18g02580, or Glyma18g02590 activity, wherein said stringent conditions comprise incubation at 65° C. in a solution comprising 6×SSC (0.9 M sodium chloride and 0.09 M sodium citrate); or(iv) a nucleotide sequence which is the reverse complement of (i), (ii), or (iii); and (c) a transcriptional termination sequence; wherein the transgenic soybean exhibits increased SCN resistance compared to a control not expressing the transcribable nucleic acid molecule.