发明名称 |
EXPRESSION-ENHANCED POLYPEPTIDES |
摘要 |
<p>A composite polypeptide, said composite polypeptide comprising a desired polypeptide and an expression enhancing domain ("EED"), said EED comprising first and second cysteine amino acid residues Cys1 and Cys2, respectively, Cys1 being located closer to the N-terminus of the composite polypeptide molecule than Cys2, wherein Cys1 and Cys2 are separated by a polypeptide linker, said linker-being free of cysteine and proline;-defining a length sufficient to allow Cys1 and Cys2 to engage in an intramolecular disulfide bond with one another; and-having a flexible polypeptide conformation essentially free of secondary polypeptide structure in aqueous solution, wherein at least one of Cys1 and Cys2 is derivatized with a derivatization moiety.</p> |
申请公布号 |
EP1769000(B1) |
申请公布日期 |
2014.12.24 |
申请号 |
EP20050774883 |
申请日期 |
2005.07.15 |
申请人 |
AMGEN RESEARCH (MUNICH) GMBH |
发明人 |
HOFFMANN, PATRICK;MITTELSTRASS, SILKE;HENNECKE, JENS;RAUM, TOBIAS |
分类号 |
C07K16/00;C07K16/46;C12N15/67 |
主分类号 |
C07K16/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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