NOVEL PURIFICATION METHOD FOR DARBEPOETIN ALPHA

摘要

The present invention relates to a high-purity purification method for darbepoetin alpha having a high content of sugar chains and, particularly, to a purification method for darbepoetin alpha comprising the steps of: (a) eluting a fraction comprising darbepoetin alpha by applying a biological emulsion comprising darbepoetin alpha to blue resin chromatography; (b) eluting a fraction comprising darbepoetin alpha by applying an eluate produced from step (a) to hydroxyapatite resin chromatography; and (c) eluting a fraction comprising darbepoetin alpha by applying an eluate produced from step (b) to anion-exchange resin chromatography.