Strains of agrobacterium modified to increase plant transformation frequency

摘要

Discloses a method for transforming a plant, comprising contacting a cell of the plant with an agrobacterium strain having at least one pTi helper plasmid comprising a 14.8 Kpnl fragment of pSB1 that is deficient in RecA function and a pTi plasmid having at least one disarmed T-DNA region, the T-DNA region comprising at least a right T-DNA border and exogenous DNA adjacent to the border, wherein the plasmids have differing origins of replication relative to each other. Also discloses the Agrobacterium and plant.