Bioengineered protein pores

摘要

A mutant Escherichia coli β-barrel monomeric protein pore bioengineered to remove a lumen-occluding domain and modify some of its extracellular domains. The modified protein, FhuA Δ/CΔ4L, forms a highly conductive pore as compared to other known pores and is capable of sensing large polypeptides and discriminating between modified protein analytes.

主权项

1. A polypeptide comprising an engineered ferric hydroxamate uptake component A (“FhuA”) from Escherichia coli, wherein said engineered FhuA is missing the cork domain and four of the eleven loop domains found in a wild-type FhuA protein, and wherein said engineered FhuA is folded into a nanopore having a stable open-state current.