Selenocysteine mediated hybrid antibody molecules

摘要

The invention provides methods and compositions employing hybrid molecules of a synthetic molecule and antibody or antibody fragment comprising a selenocysteine residue, wherein the synthetic molecule is covalently linked to the antibody or antibody fragment at the selenocysteine residue. The invention also provides a composition comprising a hybrid molecule as described above and a pharmaceutically acceptable carrier. The invention further provides for methods of making the hybrid molecules, and methods of using the hybrid molecule described above to inhibit cell surface receptor binding.

主权项

1. A composition comprising (a) a hybrid molecule comprising a synthetic molecule and an antibody or antibody fragment selected from the group consisting of Fc, F(ab′)2, Fab, scFv, IgGΔCH2, scFv2CH3, scFv4, scFv3, scFv2, dsFv, and scFv-Fc, wherein the antibody or antibody fragment comprises at least one selenocysteine residue, wherein the at least one selenocysteine residue is located within 10 amino acids of a C-terminus of the antibody or antibody fragment, wherein the at least one selenocysteine residue is cotranslationally incorporated at a UGA stop codon of the antibody or antibody fragment that was recoded from termination to selenocysteine insertion, and wherein the synthetic molecule is covalently linked to the antibody or antibody fragment at the at least one selenocysteine residue, and (b) a pharmaceutically acceptable carrier.