Metal-binding peptides

摘要

The present invention is generally directed to novel functionalized biomolecules and methods for generating such biomolecules. Biomolecules may generally include nucleic acids, peptides, multicomponent molecular complexes and/or any other molecular products that may be produced by living organisms. The present invention is further directed to cells and/or organisms manipulated to produce such functionalized biomolecules. The cells contemplated by the present invention include both prokaryotic as well as eukaryotic cells. The functionalized biomolecules are produced via materials introduced into the cell using standard molecular biology techniques or are incorporated within the genomic nucleic acid of a cell by standard recombination techniques. Further contemplated is the use of such cells for sequestration of target molecules within the cells.

主权项

1. An expression vector comprising:
a chimeric gene encoding a peptide, operatively linked to a functional promoter, said expression vector when inserted into a host transcribes said chimeric gene into a gene product which is translated into said peptide which is capable of binding to at least one target molecule which induces stress on said host, said peptide including an amino acid sequence selected from the group consisting of SEQ ID NO 1, MSHAYFVCNRCDSSNHSAHE and SEQ ID NO 2, MSHATATPASRRRLPLRS; wherein said peptide has a randomly selected peptide sequence which is not obviously biased for a specific function and is selected by sequential culturing of a host exposed to said target molecule and identifying conferred resistance to said stress on said host caused by said target molecule.