Circovirus sequences associated with piglet weight loss disease (PWD)

摘要

The genome sequences and the nucleotide sequences coding for the PWD circovirus polypeptides, such as the circovirus structural and non-structural polypeptides, vectors including the sequences, and cells and animals transformed by the vectors are provided. Methods for detecting the nucleic acids or polypeptides, and kits for diagnosing infection by a PWD circovirus, also are provided. Method for selecting compounds capable of modulating the viral infection are further provided. Pharmaceutical, including vaccine, compositions for preventing and/or treating viral infections caused by PWD circovirus and the use of vectors for preventing and/or treating diseases also are provided.

主权项

1. A method of detecting the presence of at least one antibody directed against porcine circovirus type B (PCVB) in a sample, the method comprising:
contacting the sample with a PCVB polypeptide comprising a PCVB antigen or epitope under conditions that allow the formation of an antigen-antibody complex between said polypeptide and a PCVB antibody present in the sample, wherein the polypeptide is a polypeptide having at least 90% identity to the amino acid sequence of SEQ ID NO: 26, or encoded by a nucleotide sequence with at least 90% identity to SEQ ID NO 25; and detecting the antigen-antibody complex formed.