Method for labeling of compounds

摘要

The present invention relates to site-specific labeling of antibodies or fragments thereof with one or more reporter group(s) in a way that does not affect antigen binding. The method for labeling antibodies and/or fragments thereof, comprises the following steps a) providing an IgG binding protein, which comprises α-helix structures, with a photoactivatable group and at least one label; b) forming a mixture of said IgG binding protein and the antibodies and/or fragments to be labeled; and c) UV illuminating said mixture for site-specific labeling of said antibodies and/or fragments thereof. The IgG binding protein is preferably the Z domain of Protein A.

主权项

1. A method for labeling antibody including fragments thereof, comprising:
a) providing an IgG binding protein, which comprises α-helix structures, with a photoactivatable group and at least one label and providing antibody and/or fragment thereof to be labeled; b) forming a mixture of said IgG binding protein and the antibodies and/or fragments to be labeled; and c) UV illuminating said mixture for site-specific labeling of said antibody and/or fragments thereof; wherein the IgG binding protein is a Z domain of Protein A comprising SEQ ID NO 1 in which Phe in position 5 has been exchanged for BPA (benzoylphenylalanine).