Materials and methods useful for affecting tumor cell growth, migration and invasion

摘要

It is disclosed herein that miR-221 and miR-222 down-regulate PTEN and TIMP3 tumor suppressors, resulting in TRAIL resistance. The present invention provides research, diagnostic, and therapeutic tools and methods related to this discovery. Diagnostics, prognostics and treatments for human hepatocellular cancer and non-small cell lung carcinoma having a TRAIL resistance are particularly described herein.

主权项

1. A method to reduce TNF-related apoptosis ligand (TRAIL) resistance of a cell, comprising:
transfecting the cell with at least one of: an inhibitor of miR-221 or an inhibitor of miR-222 in an amount sufficient to reduce expression of at least one of: c-Jun, miR-221 and miR-222, or to increase expression of at least one of: PTEN and TIMP3 in the cell; wherein the cell is selected from the group consisting of: a non-small cell lung carcinoma cell, and a hepatocellular carcinoma cell; and reducing TRAIL resistance of the cell.