HIV DNA vaccine regulated by a caev-derived promoter

摘要

The present invention is directed to a DNA vaccine for immunization against HIV. The invention comprises a DNA molecule that has a sequence encoding a plurality of viral proteins capable of stimulating an immune response against HIV. The DNA molecule is rendered safe for use as a vaccine by the disruption of genes encoding reverse transcriptase, integrase, and Vif. The DNA molecule is further rendered safe by at least a partial deletion of the 3′ LTR.

主权项

1. A DNA composition capable of stimulating an immune response comprising: a promoter sequence derived from Caprine Arthritis Encephalitis Goat Lentivirus (CAEV) operably linked to a coding sequence, wherein the coding sequence encodes a gag gene, a pro gene, a vpx gene, a vpr gene, a vpu gene, a nef gene, a tat gene, an env gene, and a rev gene, wherein the genes are of an immunodeficiency viral genome selected from the group consisting of Human Immunodeficiency Virus (HIV), Simian Immunodeficiency Virus (SIV), and combinations thereof, wherein the coding sequence is operably linked to a polyadenylation sequence, and wherein the DNA composition does not include functional reverse transcriptase (rt), integrase (int), and viral infectivity factor (vif).